Welcome
26
Oct
Welcome to BiotechBotany, the rarely updated personal blog of my research journey.
Taskbar separator
29
Oct
I was looking for a way to organise my Windows taskbar to make it easier for opening private vs work shortcuts. After a bit of searching, I found this perfect GitHub project: https://github.com/DrummerSi/TaskSeparator11 It does require Windows desktop runtime (6.0.24), … Read More »
Cygwin ‘Bash Prompt Here’ for Windows 11
9
May
It is always frustrating to have to navigate via bash to the working folder, so after a bit of Googling (thanks StackOverflow), this is how to set up Cygwin bash as a right-click option. Working in Windows 11. The first … Read More »
TAE (Tris/Acetic acid/EDTA) buffer
11
Feb
TAE buffer is used primarily for DNA gel electrophoresis 50x Stock Solution Component Mw(g.mol-1) 1 L Tris base (C4H11NO3) 121.14 242 g Glacial acetic acid (17.5 M) 60.05 57.1 mL 0.5 M EDTA (pH 8.0) – 100 mL dissolve 242 … Read More »
Antibiotic Stock Solutions for Bio Laboratory Use
11
Sep
Common antibiotic stock solutions for laboratory use.
Luria Broth (LB) Medium and LB Agar for E. coli growth
3
Sep
This protocol outlines preparation preparation of Luria Broth (LB) and LB Agar for plates.
Preparation of Competent Agrobacterium Cells for transformation
3
Sep
This protocol describes a method for preparing Agrobacterium for freeze-thaw transformation technique.
Freeze-Thaw Transformation of Agrobacterium tumafaciens
3
Sep
This protocol describes a method for transforming Agrobacterium with plasmid DNA using a freeze-thaw technique. Although the transformation efficiency for Agrobacterium is lower than that for E. coli, it is possible to obtain adequate numbers of transformants with this technique.
Common Stock Solutions for Bio Laboratory Use
3
Sep
A collection (from various sources) of some common stock solutions for use in bio labs.
Preparation of competent E. coli for heat-shock transformation
3
Sep
Standard protocol for preparation of competent cells for transformation with heat-shock method.
Heat-Shock Transformation of E. Coli
3
Sep
Standard protocol for Heat-Shock Transformation of E. Coli.